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plasmid 22952 deposited by  (Addgene inc)


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    Structured Review

    Addgene inc plasmid 22952 deposited by
    Plasmid 22952 Deposited By, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+22952+deposited+by/pEGFP-C1-hApg5+(Plasmid+%2322952)/pm27541856-242-33-32
    Average 93 stars, based on 7 article reviews
    plasmid 22952 deposited by - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: Full-length RAB5A was cloned into pET-41a(+) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2×FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described into vector pEGFP-N1 to create plasmid 2×FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima). .. Dominant negative GFP-RAB5A S34N and constitutive active GFP-RAB5A Q79L mutants were constructed from GFP-RAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519).

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.
    Article Snippet: Full-length RAB5A was cloned into pET-41a(C) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2£FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described44 into vector pEGFP-N1 to create plasmid 2£FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima).18 Dominant negative GFP-RAB5AS34N and constitutive active GFP-RAB5AQ79L mutants were constructed from GFPRAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519). .. RF/6A, HEK293, and DH82 cells were transfected using Fugene HD according to the manufacturer’s instructions (Promega, E2311).

    Plasmid Preparation:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: Full-length RAB5A was cloned into pET-41a(+) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2×FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described into vector pEGFP-N1 to create plasmid 2×FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima). .. Dominant negative GFP-RAB5A S34N and constitutive active GFP-RAB5A Q79L mutants were constructed from GFP-RAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519).

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.
    Article Snippet: Full-length RAB5A was cloned into pET-41a(C) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2£FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described44 into vector pEGFP-N1 to create plasmid 2£FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima).18 Dominant negative GFP-RAB5AS34N and constitutive active GFP-RAB5AQ79L mutants were constructed from GFPRAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519). .. RF/6A, HEK293, and DH82 cells were transfected using Fugene HD according to the manufacturer’s instructions (Promega, E2311).

    Expressing:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
    Article Snippet: Full-length RAB5A was cloned into pET-41a(+) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2×FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described into vector pEGFP-N1 to create plasmid 2×FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima). .. Dominant negative GFP-RAB5A S34N and constitutive active GFP-RAB5A Q79L mutants were constructed from GFP-RAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519).

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.
    Article Snippet: Full-length RAB5A was cloned into pET-41a(C) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2£FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described44 into vector pEGFP-N1 to create plasmid 2£FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima).18 Dominant negative GFP-RAB5AS34N and constitutive active GFP-RAB5AQ79L mutants were constructed from GFPRAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519). .. RF/6A, HEK293, and DH82 cells were transfected using Fugene HD according to the manufacturer’s instructions (Promega, E2311).

    Dominant Negative Mutation:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.
    Article Snippet: Full-length RAB5A was cloned into pET-41a(C) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2£FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described44 into vector pEGFP-N1 to create plasmid 2£FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima).18 Dominant negative GFP-RAB5AS34N and constitutive active GFP-RAB5AQ79L mutants were constructed from GFPRAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519). .. RF/6A, HEK293, and DH82 cells were transfected using Fugene HD according to the manufacturer’s instructions (Promega, E2311).

    Construct:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.
    Article Snippet: Full-length RAB5A was cloned into pET-41a(C) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2£FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described44 into vector pEGFP-N1 to create plasmid 2£FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima).18 Dominant negative GFP-RAB5AS34N and constitutive active GFP-RAB5AQ79L mutants were constructed from GFPRAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519). .. RF/6A, HEK293, and DH82 cells were transfected using Fugene HD according to the manufacturer’s instructions (Promega, E2311).

    Mutagenesis:

    Article Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.
    Article Snippet: Full-length RAB5A was cloned into pET-41a(C) (Novagen, 70556-3) for GST-RAB5A expression. .. A double-FYVE finger (2£FYVE) of HGS (HGF-regulated tyrosine kinase substrate) was cloned from mouse cDNA as described44 into vector pEGFP-N1 to create plasmid 2£FYVE-GFP for mammalian expression. pEGFP-C1-ATG5 (pEGFP-C1-hApg5) was obtained from Addgene (plasmid 22952, deposited by Dr. Noboru Mizushima).18 Dominant negative GFP-RAB5AS34N and constitutive active GFP-RAB5AQ79L mutants were constructed from GFPRAB5A by using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, 200519). .. RF/6A, HEK293, and DH82 cells were transfected using Fugene HD according to the manufacturer’s instructions (Promega, E2311).



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    93
    Addgene inc plasmid 22952 deposited by
    Plasmid 22952 Deposited By, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+22952+deposited+by/pEGFP-C1-hApg5+(Plasmid+%2322952)/pm27541856-242-33-32
    Average 93 stars, based on 1 article reviews
    plasmid 22952 deposited by - by Bioz Stars, 2026-10
    93/100 stars
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